Construct: shRNA TRCN0000026295

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176996.3-1306s1c1
DNA Barcode:
CCTGCGGTTATTCTCTTCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smo (319757)
Gene Description:
smoothened, frizzled class receptor
Transcript:
RefSeq NM_176996.3 (NON-CURRENT)
Match location:
Position 1306 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000026295)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCGGTTATTCTCTTCTAT
Hairpin Sequence:
5'-CCGG-CCTGCGGTTATTCTCTTCTAT-CTCGAG-ATAGAAGAGAATAACCGCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCGGTTATTCTCTTCTATCTCGAGATAGAAGAGAATAACCGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCGGTTATTCTCTTCTATCTCGAGATAGAAGAGAATAACCGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000345493