Construct: shRNA TRCN0000026297

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172812.1-2221s1c1
DNA Barcode:
CCCACTGGTATATACGTTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Htr2a (15558)
Gene Description:
5-hydroxytryptamine (serotonin) receptor 2A
Transcript:
RefSeq NM_172812.1 (NON-CURRENT)
Match location:
Position 2221 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000026297)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACTGGTATATACGTTGTT
Hairpin Sequence:
5'-CCGG-CCCACTGGTATATACGTTGTT-CTCGAG-AACAACGTATATACCAGTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACTGGTATATACGTTGTTCTCGAGAACAACGTATATACCAGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACTGGTATATACGTTGTTCTCGAGAACAACGTATATACCAGTGGG-3'

Other clones with same target sequence:

(none)