Construct: shRNA TRCN0000026519

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002225.2-449s1c1
DNA Barcode:
GACGATGCAATCAATGGGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IVD (3712)
Gene Description:
isovaleryl-CoA dehydrogenase
Transcript:
RefSeq NM_002225.2 (NON-CURRENT)
Match location:
Position 449 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000026519)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGATGCAATCAATGGGCTA
Hairpin Sequence:
5'-CCGG-GACGATGCAATCAATGGGCTA-CTCGAG-TAGCCCATTGATTGCATCGTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGATGCAATCAATGGGCTACTCGAGTAGCCCATTGATTGCATCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGATGCAATCAATGGGCTACTCGAGTAGCCCATTGATTGCATCGTC-3'

Other clones with same target sequence:

(none)