Construct: shRNA TRCN0000026925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_192925.3-1956s1c1
DNA Barcode:
CCACCTGAAATACTTTCGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itch (16396)
Gene Description:
itchy, E3 ubiquitin protein ligase
Transcript:
RefSeq XM_192925.3 (NON-CURRENT)
Match location:
Position 1956 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000026925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTGAAATACTTTCGTTT
Hairpin Sequence:
5'-CCGG-CCACCTGAAATACTTTCGTTT-CTCGAG-AAACGAAAGTATTTCAGGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTGAAATACTTTCGTTTCTCGAGAAACGAAAGTATTTCAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTGAAATACTTTCGTTTCTCGAGAAACGAAAGTATTTCAGGTGG-3'

Other clones with same target sequence:

(none)