Construct: shRNA TRCN0000026936

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_128981.2-2312s1c1
DNA Barcode:
CCAGCGTAGAAGGAAACGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Alpk2 (225638)
Gene Description:
alpha-kinase 2
Transcript:
RefSeq XM_128981.2 (NON-CURRENT)
Match location:
Position 2312 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000026936)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCGTAGAAGGAAACGAAT
Hairpin Sequence:
5'-CCGG-CCAGCGTAGAAGGAAACGAAT-CTCGAG-ATTCGTTTCCTTCTACGCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCGTAGAAGGAAACGAATCTCGAGATTCGTTTCCTTCTACGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCGTAGAAGGAAACGAATCTCGAGATTCGTTTCCTTCTACGCTGG-3'

Other clones with same target sequence:

(none)