Construct: shRNA TRCN0000026943

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013880.1-1716s1c1
DNA Barcode:
CCTCTCATCTTATGTTTAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plcl2 (224860)
Gene Description:
phospholipase C-like 2
Transcript:
RefSeq NM_013880.1 (NON-CURRENT)
Match location:
Position 1716 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000026943)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCATCTTATGTTTAGAA
Hairpin Sequence:
5'-CCGG-CCTCTCATCTTATGTTTAGAA-CTCGAG-TTCTAAACATAAGATGAGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCATCTTATGTTTAGAACTCGAGTTCTAAACATAAGATGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCATCTTATGTTTAGAACTCGAGTTCTAAACATAAGATGAGAGG-3'

Other clones with same target sequence:

  1. TRCN0000321673