Construct: shRNA TRCN0000026947

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177898.3-1430s1c1
DNA Barcode:
CCAGAAGCAGAGGGTTTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nek5 (330721)
Gene Description:
NIMA (never in mitosis gene a)-related expressed kinase 5
Transcript:
RefSeq NM_177898.3 (NON-CURRENT)
Match location:
Position 1430 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000026947)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAAGCAGAGGGTTTCTTT
Hairpin Sequence:
5'-CCGG-CCAGAAGCAGAGGGTTTCTTT-CTCGAG-AAAGAAACCCTCTGCTTCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAAGCAGAGGGTTTCTTTCTCGAGAAAGAAACCCTCTGCTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAAGCAGAGGGTTTCTTTCTCGAGAAAGAAACCCTCTGCTTCTGG-3'

Other clones with same target sequence:

(none)