Construct: shRNA TRCN0000026994

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023815.2-652s1c1
DNA Barcode:
CGCGTTTGAAGCCTTTCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trp53rkb (76367)
Gene Description:
transformation related protein 53 regulating kinase B
Transcript:
RefSeq NM_023815.2 (NON-CURRENT)
Match location:
Position 652 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000026994)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGTTTGAAGCCTTTCTGAA
Hairpin Sequence:
5'-CCGG-CGCGTTTGAAGCCTTTCTGAA-CTCGAG-TTCAGAAAGGCTTCAAACGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGTTTGAAGCCTTTCTGAACTCGAGTTCAGAAAGGCTTCAAACGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGTTTGAAGCCTTTCTGAACTCGAGTTCAGAAAGGCTTCAAACGCG-3'

Other clones with same target sequence:

(none)