Construct: shRNA TRCN0000027010

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080708.1-2284s1c1
DNA Barcode:
CGGAACATTCTCCAAATCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bmp2k (140780)
Gene Description:
BMP2 inducible kinase
Transcript:
RefSeq NM_080708.1 (CURRENT)
Match location:
Position 2284 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027010)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAACATTCTCCAAATCAAA
Hairpin Sequence:
5'-CCGG-CGGAACATTCTCCAAATCAAA-CTCGAG-TTTGATTTGGAGAATGTTCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAACATTCTCCAAATCAAACTCGAGTTTGATTTGGAGAATGTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAACATTCTCCAAATCAAACTCGAGTTTGATTTGGAGAATGTTCCG-3'

Other clones with same target sequence:

(none)