Construct: shRNA TRCN0000027021

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021606.2-1005s1c1
DNA Barcode:
CACCGACCTGACATTGTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nek6 (59126)
Gene Description:
NIMA (never in mitosis gene a)-related expressed kinase 6
Transcript:
RefSeq NM_021606.2 (NON-CURRENT)
Match location:
Position 1005 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027021)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCGACCTGACATTGTATAT
Hairpin Sequence:
5'-CCGG-CACCGACCTGACATTGTATAT-CTCGAG-ATATACAATGTCAGGTCGGTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCGACCTGACATTGTATATCTCGAGATATACAATGTCAGGTCGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCGACCTGACATTGTATATCTCGAGATATACAATGTCAGGTCGGTG-3'

Other clones with same target sequence:

  1. TRCN0000274724