Construct: shRNA TRCN0000027023

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198703.1-2079s1c1
DNA Barcode:
GCAACCTAGTATATCTGTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wnk1 (232341)
Gene Description:
WNK lysine deficient protein kinase 1
Transcript:
RefSeq NM_198703.1 (NON-CURRENT)
Match location:
Position 2079 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027023)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACCTAGTATATCTGTGTT
Hairpin Sequence:
5'-CCGG-GCAACCTAGTATATCTGTGTT-CTCGAG-AACACAGATATACTAGGTTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACCTAGTATATCTGTGTTCTCGAGAACACAGATATACTAGGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACCTAGTATATCTGTGTTCTCGAGAACACAGATATACTAGGTTGC-3'

Other clones with same target sequence:

  1. TRCN0000345023