Construct: shRNA TRCN0000027041

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011163.2-894s1c1
DNA Barcode:
CGCCAGGTTTAACAGCGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif2ak2 (19106)
Gene Description:
eukaryotic translation initiation factor 2-alpha kinase 2
Transcript:
RefSeq NM_011163.2 (NON-CURRENT)
Match location:
Position 894 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027041)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCAGGTTTAACAGCGATTT
Hairpin Sequence:
5'-CCGG-CGCCAGGTTTAACAGCGATTT-CTCGAG-AAATCGCTGTTAAACCTGGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCAGGTTTAACAGCGATTTCTCGAGAAATCGCTGTTAAACCTGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCAGGTTTAACAGCGATTTCTCGAGAAATCGCTGTTAAACCTGGCG-3'

Other clones with same target sequence:

  1. TRCN0000274651