Construct: shRNA TRCN0000027168

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004544.2-990s1c1
DNA Barcode:
GTGCTGAATTACACAAGCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NDUFA10 (4705)
Gene Description:
NADH:ubiquinone oxidoreductase subunit A10
Transcript:
RefSeq NM_004544.2 (NON-CURRENT)
Match location:
Position 990 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027168)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCTGAATTACACAAGCATT
Hairpin Sequence:
5'-CCGG-GTGCTGAATTACACAAGCATT-CTCGAG-AATGCTTGTGTAATTCAGCAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCTGAATTACACAAGCATTCTCGAGAATGCTTGTGTAATTCAGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCTGAATTACACAAGCATTCTCGAGAATGCTTGTGTAATTCAGCAC-3'

Other clones with same target sequence:

(none)