Construct: shRNA TRCN0000027183

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000693.1-1535s1c1
DNA Barcode:
GCCGAATACACAGAAGTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALDH1A3 (220)
Gene Description:
aldehyde dehydrogenase 1 family member A3
Transcript:
RefSeq NM_000693.1 (NON-CURRENT)
Match location:
Position 1535 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027183)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAATACACAGAAGTGAAA
Hairpin Sequence:
5'-CCGG-GCCGAATACACAGAAGTGAAA-CTCGAG-TTTCACTTCTGTGTATTCGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAATACACAGAAGTGAAACTCGAGTTTCACTTCTGTGTATTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAATACACAGAAGTGAAACTCGAGTTTCACTTCTGTGTATTCGGC-3'

Other clones with same target sequence:

(none)