Construct: shRNA TRCN0000027207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005002.3-362s1c1
DNA Barcode:
CGAGTAGTACAACACAGCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NDUFA9 (4704)
Gene Description:
NADH:ubiquinone oxidoreductase subunit A9
Transcript:
RefSeq NM_005002.3 (NON-CURRENT)
Match location:
Position 362 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGTAGTACAACACAGCAAT
Hairpin Sequence:
5'-CCGG-CGAGTAGTACAACACAGCAAT-CTCGAG-ATTGCTGTGTTGTACTACTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGTAGTACAACACAGCAATCTCGAGATTGCTGTGTTGTACTACTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGTAGTACAACACAGCAATCTCGAGATTGCTGTGTTGTACTACTCG-3'

Other clones with same target sequence:

  1. TRCN0000338672