Construct: shRNA TRCN0000027341

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033269.2-1052s1c1
DNA Barcode:
GTGGCATTTAAGGTCAACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chrm3 (12671)
Gene Description:
cholinergic receptor, muscarinic 3, cardiac
Transcript:
RefSeq NM_033269.2 (NON-CURRENT)
Match location:
Position 1052 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027341)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGCATTTAAGGTCAACAAA
Hairpin Sequence:
5'-CCGG-GTGGCATTTAAGGTCAACAAA-CTCGAG-TTTGTTGACCTTAAATGCCAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGCATTTAAGGTCAACAAACTCGAGTTTGTTGACCTTAAATGCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGCATTTAAGGTCAACAAACTCGAGTTTGTTGACCTTAAATGCCAC-3'

Other clones with same target sequence:

(none)