Construct: shRNA TRCN0000027364

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007407.2-1515s1c1
DNA Barcode:
CGTGCAGAAATGCTACTGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adcyap1r1 (11517)
Gene Description:
adenylate cyclase activating polypeptide 1 receptor 1
Transcript:
RefSeq NM_007407.2 (NON-CURRENT)
Match location:
Position 1515 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027364)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGCAGAAATGCTACTGCAA
Hairpin Sequence:
5'-CCGG-CGTGCAGAAATGCTACTGCAA-CTCGAG-TTGCAGTAGCATTTCTGCACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGCAGAAATGCTACTGCAACTCGAGTTGCAGTAGCATTTCTGCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGCAGAAATGCTACTGCAACTCGAGTTGCAGTAGCATTTCTGCACG-3'

Other clones with same target sequence:

(none)