Construct: shRNA TRCN0000027366

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009886.1-4952s1c1
DNA Barcode:
GCTGCGTTTATTGCCAACAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Celsr1 (12614)
Gene Description:
cadherin, EGF LAG seven-pass G-type receptor 1
Transcript:
RefSeq NM_009886.1 (NON-CURRENT)
Match location:
Position 4952 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027366)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCGTTTATTGCCAACAAT
Hairpin Sequence:
5'-CCGG-GCTGCGTTTATTGCCAACAAT-CTCGAG-ATTGTTGGCAATAAACGCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCGTTTATTGCCAACAATCTCGAGATTGTTGGCAATAAACGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCGTTTATTGCCAACAATCTCGAGATTGTTGGCAATAAACGCAGC-3'

Other clones with same target sequence:

(none)