Construct: shRNA TRCN0000027387

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007416.1-1258s1c1
DNA Barcode:
CCTCTCCTTGGATGGAAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adra1b (11548)
Gene Description:
adrenergic receptor, alpha 1b
Transcript:
RefSeq NM_007416.1 (NON-CURRENT)
Match location:
Position 1258 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027387)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCCTTGGATGGAAAGAA
Hairpin Sequence:
5'-CCGG-CCTCTCCTTGGATGGAAAGAA-CTCGAG-TTCTTTCCATCCAAGGAGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCCTTGGATGGAAAGAACTCGAGTTCTTTCCATCCAAGGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCCTTGGATGGAAAGAACTCGAGTTCTTTCCATCCAAGGAGAGG-3'

Other clones with same target sequence:

(none)