Construct: shRNA TRCN0000027410

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139138.1-1367s1c1
DNA Barcode:
GCTTCCTTCATGTGGATGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgre4 (52614)
Gene Description:
adhesion G protein-coupled receptor E4
Transcript:
RefSeq NM_139138.1 (NON-CURRENT)
Match location:
Position 1367 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027410)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCCTTCATGTGGATGTTT
Hairpin Sequence:
5'-CCGG-GCTTCCTTCATGTGGATGTTT-CTCGAG-AAACATCCACATGAAGGAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCCTTCATGTGGATGTTTCTCGAGAAACATCCACATGAAGGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCCTTCATGTGGATGTTTCTCGAGAAACATCCACATGAAGGAAGC-3'

Other clones with same target sequence:

(none)