Construct: shRNA TRCN0000027479

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173410.1-1222s1c1
DNA Barcode:
CTTGTGTGCTTTGCACCCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr26 (233919)
Gene Description:
G protein-coupled receptor 26
Transcript:
RefSeq NM_173410.1 (NON-CURRENT)
Match location:
Position 1222 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027479)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGTGTGCTTTGCACCCTAT
Hairpin Sequence:
5'-CCGG-CTTGTGTGCTTTGCACCCTAT-CTCGAG-ATAGGGTGCAAAGCACACAAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGTGTGCTTTGCACCCTATCTCGAGATAGGGTGCAAAGCACACAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGTGTGCTTTGCACCCTATCTCGAGATAGGGTGCAAAGCACACAAG-3'

Other clones with same target sequence:

(none)