Construct: shRNA TRCN0000027563

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007420.2-1680s1c1
DNA Barcode:
CCTCTTTGTATGGAACTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adrb2 (11555)
Gene Description:
adrenergic receptor, beta 2
Transcript:
RefSeq NM_007420.2 (NON-CURRENT)
Match location:
Position 1680 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027563)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTTTGTATGGAACTTAAA
Hairpin Sequence:
5'-CCGG-CCTCTTTGTATGGAACTTAAA-CTCGAG-TTTAAGTTCCATACAAAGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTTTGTATGGAACTTAAACTCGAGTTTAAGTTCCATACAAAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTTTGTATGGAACTTAAACTCGAGTTTAAGTTCCATACAAAGAGG-3'

Other clones with same target sequence:

(none)