Construct: shRNA TRCN0000027590

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_127323.4-4498s1c1
DNA Barcode:
CCCTCCTGTGACTACTGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wnk2 (75607)
Gene Description:
WNK lysine deficient protein kinase 2
Transcript:
RefSeq XM_127323.4 (NON-CURRENT)
Match location:
Position 4498 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027590)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCCTGTGACTACTGTAAT
Hairpin Sequence:
5'-CCGG-CCCTCCTGTGACTACTGTAAT-CTCGAG-ATTACAGTAGTCACAGGAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCCTGTGACTACTGTAATCTCGAGATTACAGTAGTCACAGGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCCTGTGACTACTGTAATCTCGAGATTACAGTAGTCACAGGAGGG-3'

Other clones with same target sequence:

(none)