Construct: shRNA TRCN0000027605

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_132229.5-3411s1c1
DNA Barcode:
CTTCTCTAAGTCTGACAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gak (231580)
Gene Description:
cyclin G associated kinase
Transcript:
RefSeq XM_132229.5 (NON-CURRENT)
Match location:
Position 3411 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027605)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTCTAAGTCTGACAAGAA
Hairpin Sequence:
5'-CCGG-CTTCTCTAAGTCTGACAAGAA-CTCGAG-TTCTTGTCAGACTTAGAGAAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTCTAAGTCTGACAAGAACTCGAGTTCTTGTCAGACTTAGAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTCTAAGTCTGACAAGAACTCGAGTTCTTGTCAGACTTAGAGAAG-3'

Other clones with same target sequence:

(none)