Construct: shRNA TRCN0000027614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_142224.3-1736s1c1
DNA Barcode:
CCAGTTGATTAAAGGACCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gucy2f (245650)
Gene Description:
guanylate cyclase 2f
Transcript:
RefSeq XM_142224.3 (NON-CURRENT)
Match location:
Position 1736 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTTGATTAAAGGACCCAA
Hairpin Sequence:
5'-CCGG-CCAGTTGATTAAAGGACCCAA-CTCGAG-TTGGGTCCTTTAATCAACTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTTGATTAAAGGACCCAACTCGAGTTGGGTCCTTTAATCAACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTTGATTAAAGGACCCAACTCGAGTTGGGTCCTTTAATCAACTGG-3'

Other clones with same target sequence:

(none)