Construct: shRNA TRCN0000027615

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010892.1-187s1c1
DNA Barcode:
GTGTGGAAAGAGCTTGACTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nek2 (18005)
Gene Description:
NIMA (never in mitosis gene a)-related expressed kinase 2
Transcript:
RefSeq NM_010892.1 (NON-CURRENT)
Match location:
Position 187 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027615)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTGGAAAGAGCTTGACTAT
Hairpin Sequence:
5'-CCGG-GTGTGGAAAGAGCTTGACTAT-CTCGAG-ATAGTCAAGCTCTTTCCACAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTGGAAAGAGCTTGACTATCTCGAGATAGTCAAGCTCTTTCCACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTGGAAAGAGCTTGACTATCTCGAGATAGTCAAGCTCTTTCCACAC-3'

Other clones with same target sequence:

  1. TRCN0000285711