Construct: shRNA TRCN0000027621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020572.1-967s1c1
DNA Barcode:
CCGGAAATGATAGCGCAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aurkc (20871)
Gene Description:
aurora kinase C
Transcript:
RefSeq NM_020572.1 (NON-CURRENT)
Match location:
Position 967 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAAATGATAGCGCAGAAA
Hairpin Sequence:
5'-CCGG-CCGGAAATGATAGCGCAGAAA-CTCGAG-TTTCTGCGCTATCATTTCCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAAATGATAGCGCAGAAACTCGAGTTTCTGCGCTATCATTTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAAATGATAGCGCAGAAACTCGAGTTTCTGCGCTATCATTTCCGG-3'

Other clones with same target sequence:

(none)