Construct: shRNA TRCN0000027693

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355649.1-922s1c1
DNA Barcode:
CGGAATGACTTTCTAGGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC381675 (381675)
Gene Description:
similar to Ca2+-promoted Ras inactivator; GTPase activating protein-like; calcium-promoted Ras inactivator
Transcript:
RefSeq XM_355649.1 (NON-CURRENT)
Match location:
Position 922 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027693)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAATGACTTTCTAGGCAAA
Hairpin Sequence:
5'-CCGG-CGGAATGACTTTCTAGGCAAA-CTCGAG-TTTGCCTAGAAAGTCATTCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAATGACTTTCTAGGCAAACTCGAGTTTGCCTAGAAAGTCATTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAATGACTTTCTAGGCAAACTCGAGTTTGCCTAGAAAGTCATTCCG-3'

Other clones with same target sequence:

(none)