Construct: shRNA TRCN0000027696

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_110396.3-3781s1c1
DNA Barcode:
GCGGAGTTTGAACTGCTAACT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fer1l4 (74562)
Gene Description:
fer-1-like 4 (C. elegans)
Transcript:
RefSeq XM_110396.3 (NON-CURRENT)
Match location:
Position 3781 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027696)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAGTTTGAACTGCTAACT
Hairpin Sequence:
5'-CCGG-GCGGAGTTTGAACTGCTAACT-CTCGAG-AGTTAGCAGTTCAAACTCCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAGTTTGAACTGCTAACTCTCGAGAGTTAGCAGTTCAAACTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAGTTTGAACTGCTAACTCTCGAGAGTTAGCAGTTCAAACTCCGC-3'

Other clones with same target sequence:

(none)