Construct: shRNA TRCN0000027702

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_129785.3-1286s1c1
DNA Barcode:
CCCTACTCTGAAGGAATCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plcl1 (227120)
Gene Description:
phospholipase C-like 1
Transcript:
RefSeq XM_129785.3 (NON-CURRENT)
Match location:
Position 1286 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027702)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTACTCTGAAGGAATCTAA
Hairpin Sequence:
5'-CCGG-CCCTACTCTGAAGGAATCTAA-CTCGAG-TTAGATTCCTTCAGAGTAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTACTCTGAAGGAATCTAACTCGAGTTAGATTCCTTCAGAGTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTACTCTGAAGGAATCTAACTCGAGTTAGATTCCTTCAGAGTAGGG-3'

Other clones with same target sequence:

(none)