Construct: shRNA TRCN0000027727
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_129857.4-1002s1c1
- DNA Barcode:
- GAAGAGTGATTGTTGCCACAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Tgfbrap1 (73122)
- Gene Description:
- transforming growth factor, beta receptor associated protein 1
- Transcript:
- RefSeq XM_129857.4 (NON-CURRENT)
- Match location:
- Position 1002 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000027727)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAAGAGTGATTGTTGCCACAA
- Hairpin Sequence:
-
5'-CCGG-GAAGAGTGATTGTTGCCACAA-CTCGAG-TTGTGGCAACAATCACTCTTC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAAGAGTGATTGTTGCCACAACTCGAGTTGTGGCAACAATCACTCTTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAAGAGTGATTGTTGCCACAACTCGAGTTGTGGCAACAATCACTCTTC-3'
Other clones with same target sequence:
(none)
