Construct: shRNA TRCN0000027752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010816.1-1799s1c1
DNA Barcode:
GCCGCAGAAGTCTCAACTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Morc1 (17450)
Gene Description:
microrchidia 1
Transcript:
RefSeq NM_010816.1 (CURRENT)
Match location:
Position 1799 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGCAGAAGTCTCAACTTAT
Hairpin Sequence:
5'-CCGG-GCCGCAGAAGTCTCAACTTAT-CTCGAG-ATAAGTTGAGACTTCTGCGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGCAGAAGTCTCAACTTATCTCGAGATAAGTTGAGACTTCTGCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGCAGAAGTCTCAACTTATCTCGAGATAAGTTGAGACTTCTGCGGC-3'

Other clones with same target sequence:

(none)