Construct: shRNA TRCN0000027945

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139270.1-262s1c1
DNA Barcode:
CCGTATGTTTACGACTTCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pth2r (213527)
Gene Description:
parathyroid hormone 2 receptor
Transcript:
RefSeq NM_139270.1 (NON-CURRENT)
Match location:
Position 262 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027945)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTATGTTTACGACTTCAAT
Hairpin Sequence:
5'-CCGG-CCGTATGTTTACGACTTCAAT-CTCGAG-ATTGAAGTCGTAAACATACGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTATGTTTACGACTTCAATCTCGAGATTGAAGTCGTAAACATACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTATGTTTACGACTTCAATCTCGAGATTGAAGTCGTAAACATACGG-3'

Other clones with same target sequence:

(none)