Construct: shRNA TRCN0000027985

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011199.1-877s1c1
DNA Barcode:
GCTACCAACTACTACTGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pth1r (19228)
Gene Description:
parathyroid hormone 1 receptor
Transcript:
RefSeq NM_011199.1 (NON-CURRENT)
Match location:
Position 877 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027985)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACCAACTACTACTGGATT
Hairpin Sequence:
5'-CCGG-GCTACCAACTACTACTGGATT-CTCGAG-AATCCAGTAGTAGTTGGTAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACCAACTACTACTGGATTCTCGAGAATCCAGTAGTAGTTGGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACCAACTACTACTGGATTCTCGAGAATCCAGTAGTAGTTGGTAGC-3'

Other clones with same target sequence:

(none)