Construct: shRNA TRCN0000027999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018882.2-383s1c1
DNA Barcode:
GCAAGCATGACTACCTGCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgrg1 (14766)
Gene Description:
adhesion G protein-coupled receptor G1
Transcript:
RefSeq NM_018882.2 (NON-CURRENT)
Match location:
Position 383 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000027999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGCATGACTACCTGCTTA
Hairpin Sequence:
5'-CCGG-GCAAGCATGACTACCTGCTTA-CTCGAG-TAAGCAGGTAGTCATGCTTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGCATGACTACCTGCTTACTCGAGTAAGCAGGTAGTCATGCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGCATGACTACCTGCTTACTCGAGTAAGCAGGTAGTCATGCTTGC-3'

Other clones with same target sequence:

  1. TRCN0000279066