Construct: shRNA TRCN0000028019

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009492.1-2488s1c1
DNA Barcode:
CCACAAAGGTAAATTGCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn2r122 (22308)
Gene Description:
vomeronasal 2, receptor, 122
Transcript:
RefSeq NM_009492.1 (NON-CURRENT)
Match location:
Position 2488 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000028019)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAAAGGTAAATTGCTTTA
Hairpin Sequence:
5'-CCGG-CCACAAAGGTAAATTGCTTTA-CTCGAG-TAAAGCAATTTACCTTTGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAAAGGTAAATTGCTTTACTCGAGTAAAGCAATTTACCTTTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAAAGGTAAATTGCTTTACTCGAGTAAAGCAATTTACCTTTGTGG-3'

Other clones with same target sequence:

(none)