Construct: shRNA TRCN0000028032

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019945.1-945s1c1
DNA Barcode:
CCAGAAGAGTTCTACCACCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mast1 (56527)
Gene Description:
microtubule associated serine/threonine kinase 1
Transcript:
RefSeq NM_019945.1 (NON-CURRENT)
Match location:
Position 945 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000028032)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAAGAGTTCTACCACCTT
Hairpin Sequence:
5'-CCGG-CCAGAAGAGTTCTACCACCTT-CTCGAG-AAGGTGGTAGAACTCTTCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAAGAGTTCTACCACCTTCTCGAGAAGGTGGTAGAACTCTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAAGAGTTCTACCACCTTCTCGAGAAGGTGGTAGAACTCTTCTGG-3'

Other clones with same target sequence:

(none)