Construct: shRNA TRCN0000028224

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_356087.1-1815s1c1
DNA Barcode:
GCCTGAGATCTTCGAGCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Unc13a (382018)
Gene Description:
unc-13 homolog A (C. elegans)
Transcript:
RefSeq XM_356087.1 (NON-CURRENT)
Match location:
Position 1815 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000028224)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGAGATCTTCGAGCTTAT
Hairpin Sequence:
5'-CCGG-GCCTGAGATCTTCGAGCTTAT-CTCGAG-ATAAGCTCGAAGATCTCAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGAGATCTTCGAGCTTATCTCGAGATAAGCTCGAAGATCTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGAGATCTTCGAGCTTATCTCGAGATAAGCTCGAAGATCTCAGGC-3'

Other clones with same target sequence:

(none)