Construct: shRNA TRCN0000028265

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_356816.1-3156s1c1
DNA Barcode:
CCTGATTTATGACCATGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC383041 (383041)
Gene Description:
similar to otoferlin isoform c
Transcript:
RefSeq XM_356816.1 (NON-CURRENT)
Match location:
Position 3156 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000028265)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATTTATGACCATGACAT
Hairpin Sequence:
5'-CCGG-CCTGATTTATGACCATGACAT-CTCGAG-ATGTCATGGTCATAAATCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATTTATGACCATGACATCTCGAGATGTCATGGTCATAAATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATTTATGACCATGACATCTCGAGATGTCATGGTCATAAATCAGG-3'

Other clones with same target sequence:

(none)