Construct: shRNA TRCN0000028270

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_127419.3-767s1c1
DNA Barcode:
CCGGAATGTACCAGTTGGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mctp1 (78771)
Gene Description:
multiple C2 domains, transmembrane 1
Transcript:
RefSeq XM_127419.3 (NON-CURRENT)
Match location:
Position 767 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000028270)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAATGTACCAGTTGGATA
Hairpin Sequence:
5'-CCGG-CCGGAATGTACCAGTTGGATA-CTCGAG-TATCCAACTGGTACATTCCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAATGTACCAGTTGGATACTCGAGTATCCAACTGGTACATTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAATGTACCAGTTGGATACTCGAGTATCCAACTGGTACATTCCGG-3'

Other clones with same target sequence:

(none)