Construct: shRNA TRCN0000028455

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004547.4-137s1c1
DNA Barcode:
CCAGCCGAATACAACATATCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NDUFB4 (4710)
Gene Description:
NADH:ubiquinone oxidoreductase subunit B4
Transcript:
RefSeq NM_004547.4 (NON-CURRENT)
Match location:
Position 137 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000028455)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCCGAATACAACATATCT
Hairpin Sequence:
5'-CCGG-CCAGCCGAATACAACATATCT-CTCGAG-AGATATGTTGTATTCGGCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCCGAATACAACATATCTCTCGAGAGATATGTTGTATTCGGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCCGAATACAACATATCTCTCGAGAGATATGTTGTATTCGGCTGG-3'

Other clones with same target sequence:

(none)