Construct: shRNA TRCN0000028468

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183050.1-1086s1c1
DNA Barcode:
GAGGAATGTTTCTTGAACCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCKDHB (594)
Gene Description:
branched chain keto acid dehydrogenase E1 subunit beta
Transcript:
RefSeq NM_183050.1 (NON-CURRENT)
Match location:
Position 1086 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000028468)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAATGTTTCTTGAACCTA
Hairpin Sequence:
5'-CCGG-GAGGAATGTTTCTTGAACCTA-CTCGAG-TAGGTTCAAGAAACATTCCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAATGTTTCTTGAACCTACTCGAGTAGGTTCAAGAAACATTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAATGTTTCTTGAACCTACTCGAGTAGGTTCAAGAAACATTCCTC-3'

Other clones with same target sequence:

(none)