Construct: shRNA TRCN0000028708

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027571.2-598s1c1
DNA Barcode:
CCGCAGTAAATCCAACTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
P2ry12 (70839)
Gene Description:
purinergic receptor P2Y, G-protein coupled 12
Transcript:
RefSeq NM_027571.2 (NON-CURRENT)
Match location:
Position 598 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000028708)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCAGTAAATCCAACTTCAT
Hairpin Sequence:
5'-CCGG-CCGCAGTAAATCCAACTTCAT-CTCGAG-ATGAAGTTGGATTTACTGCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCAGTAAATCCAACTTCATCTCGAGATGAAGTTGGATTTACTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCAGTAAATCCAACTTCATCTCGAGATGAAGTTGGATTTACTGCGG-3'

Other clones with same target sequence:

(none)