Construct: shRNA TRCN0000028718

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133776.1-2221s1c1
DNA Barcode:
GCTGATGTTTGGTTTATCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgrf1 (77596)
Gene Description:
adhesion G protein-coupled receptor F1
Transcript:
RefSeq NM_133776.1 (NON-CURRENT)
Match location:
Position 2221 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000028718)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATGTTTGGTTTATCATT
Hairpin Sequence:
5'-CCGG-GCTGATGTTTGGTTTATCATT-CTCGAG-AATGATAAACCAAACATCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATGTTTGGTTTATCATTCTCGAGAATGATAAACCAAACATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATGTTTGGTTTATCATTCTCGAGAATGATAAACCAAACATCAGC-3'

Other clones with same target sequence:

(none)