Construct: shRNA TRCN0000028760

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_181344.3-531s1c1
DNA Barcode:
GCAGCCTTTCACTATTGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aurkb (20877)
Gene Description:
aurora kinase B
Transcript:
RefSeq XM_181344.3 (NON-CURRENT)
Match location:
Position 531 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000028760)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCCTTTCACTATTGACAA
Hairpin Sequence:
5'-CCGG-GCAGCCTTTCACTATTGACAA-CTCGAG-TTGTCAATAGTGAAAGGCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCCTTTCACTATTGACAACTCGAGTTGTCAATAGTGAAAGGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCCTTTCACTATTGACAACTCGAGTTGTCAATAGTGAAAGGCTGC-3'

Other clones with same target sequence:

(none)