Construct: shRNA TRCN0000028777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021896.3-971s1c1
DNA Barcode:
CCCTATTTGCTCTACTCCGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gucy1a3 (60596)
Gene Description:
guanylate cyclase 1, soluble, alpha 3
Transcript:
RefSeq NM_021896.3 (NON-CURRENT)
Match location:
Position 971 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000028777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTATTTGCTCTACTCCGTT
Hairpin Sequence:
5'-CCGG-CCCTATTTGCTCTACTCCGTT-CTCGAG-AACGGAGTAGAGCAAATAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTATTTGCTCTACTCCGTTCTCGAGAACGGAGTAGAGCAAATAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTATTTGCTCTACTCCGTTCTCGAGAACGGAGTAGAGCAAATAGGG-3'

Other clones with same target sequence:

(none)