Construct: shRNA TRCN0000028936

Construct Description:

Construct Type:
shRNA
Other Identifiers:
ACCA_mouse_AY451393.1-841s1c1
DNA Barcode:
CCACAGGATCTGTATGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acaca (107476)
Gene Description:
acetyl-Coenzyme A carboxylase alpha
Transcript:
BMS ACCA_mouse_AY451393.1 (NON-CURRENT)
Match location:
Position 841 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000028936)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGGATCTGTATGAGAAA
Hairpin Sequence:
5'-CCGG-CCACAGGATCTGTATGAGAAA-CTCGAG-TTTCTCATACAGATCCTGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGGATCTGTATGAGAAACTCGAGTTTCTCATACAGATCCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGGATCTGTATGAGAAACTCGAGTTTCTCATACAGATCCTGTGG-3'

Other clones with same target sequence:

(none)