Construct: shRNA TRCN0000029016

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008976.1-3272s1c1
DNA Barcode:
CGGACAGATTCTGGTTGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptpn14 (19250)
Gene Description:
protein tyrosine phosphatase, non-receptor type 14
Transcript:
RefSeq NM_008976.1 (NON-CURRENT)
Match location:
Position 3272 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029016)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACAGATTCTGGTTGCTAT
Hairpin Sequence:
5'-CCGG-CGGACAGATTCTGGTTGCTAT-CTCGAG-ATAGCAACCAGAATCTGTCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACAGATTCTGGTTGCTATCTCGAGATAGCAACCAGAATCTGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACAGATTCTGGTTGCTATCTCGAGATAGCAACCAGAATCTGTCCG-3'

Other clones with same target sequence:

(none)