Construct: shRNA TRCN0000029035

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003019.3-894s1c1
DNA Barcode:
CCAGGCTGCTTTCTCTCAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SFTPD (6441)
Gene Description:
surfactant protein D
Transcript:
RefSeq NM_003019.3 (NON-CURRENT)
Match location:
Position 894 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029035)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGCTGCTTTCTCTCAGTA
Hairpin Sequence:
5'-CCGG-CCAGGCTGCTTTCTCTCAGTA-CTCGAG-TACTGAGAGAAAGCAGCCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGCTGCTTTCTCTCAGTACTCGAGTACTGAGAGAAAGCAGCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGCTGCTTTCTCTCAGTACTCGAGTACTGAGAGAAAGCAGCCTGG-3'

Other clones with same target sequence:

(none)