Construct: shRNA TRCN0000029089

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005502.2-406s1c1
DNA Barcode:
GCCTCTATTTATCTTCCTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABCA1 (19)
Gene Description:
ATP binding cassette subfamily A member 1
Transcript:
RefSeq NM_005502.2 (NON-CURRENT)
Match location:
Position 406 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029089)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTATTTATCTTCCTGAT
Hairpin Sequence:
5'-CCGG-GCCTCTATTTATCTTCCTGAT-CTCGAG-ATCAGGAAGATAAATAGAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTATTTATCTTCCTGATCTCGAGATCAGGAAGATAAATAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTATTTATCTTCCTGATCTCGAGATCAGGAAGATAAATAGAGGC-3'

Other clones with same target sequence:

  1. TRCN0000276419